Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (ER1907-23) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (ER1907-23) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: Hela Cell Lysate at 40 ug Hcclm3 Cell Lysate at 40 ug Primary: Anti-DC-SIGN (ER1907-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kDApplication
Fig4: Blank control (blue line): MCF7 (blue). Primary Antibody (green line): Rabbit Anti-DC-SIGN antibody（ER1907-23） Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC. Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | DC-SIGN |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLEC4L Dendritic cell-specific ICAM-3-grabbing non-integrin 1 C type lectin domain family 4 member L CD 209 CD209 CD209 antigen CD209 antigen-like protein A CD209 molecule Cd209a CDSIGN CIRE DC SIGN1 DCSIGN Dendritic cell specific ICAM 3 grabbing nonintegrin 1 Dendritic cell specific ICAM3 grabbing nonintegrin 1 Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin Dengue fever, protection against, included Dentritic Cell Specific ICAM3 Grabbing Nonintegrin HIV GP120 Binding Protein MGC129965 MGC130443 SIGN-R1 SIGNR5 CD209_HUMAN. |
| Product description | Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. ;On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human DC-SIGN/CD209 51-150/1404 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLEC4L
Dendritic cell-specific ICAM-3-grabbing non-integrin 1
C type lectin domain family 4 member L
CD 209
CD209
CD209 antigen
CD209 antigen-like protein A
CD209 molecule
Cd209a
CDSIGN
CIRE
DC SIGN1
DCSIGN
Dendritic cell specific ICAM 3 grabbing nonintegrin 1
Dendritic cell specific ICAM3 grabbing nonintegrin 1
Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin
Dengue fever protection against included Dentritic Cell Specific ICAM3 Grabbing Nonintegrin HIV GP120 Binding Protein MGC129965 MGC130443 SIGN-R1 SIGNR5 CD209_HUMAN. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| Cellular Localization | Isoform 1, 2, 3, 4, 5, : Cell membrane; Single-pass type II membrane protein (Probable). Isoform 6, 7, 8, 9, 10, 11, 12: Secreted (Probable). |
| SwissProt ID | Q9NNX6 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (ER1907-23) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (ER1907-23) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: Hela Cell Lysate at 40 ug Hcclm3 Cell Lysate at 40 ug Primary: Anti-DC-SIGN (ER1907-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD
Application
Fig4: Blank control (blue line): MCF7 (blue). Primary Antibody (green line): Rabbit Anti-DC-SIGN antibody（ER1907-23） Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC. Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
