Application
Fig1: Sample:Pancreas (Mouse) Lysate at 40 ug Primary: Anti-DC-SIGNR1/CD209b(ER1907-24)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 37kDApplication
Fig2: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-DC-SIGNR1/CD209b(ER1907-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD| Product Name | DC-SIGNR1/CD209b |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIGN Related 1 CD209 antigen like protein B CD209b antigen DC SIGN related protein 1 DC SIGNR1 OtB7. |
| Product description | SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD209b 51-150/325 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Alternative Names | SIGN Related 1 CD209 antigen like protein B CD209b antigen DC SIGN related protein 1 DC SIGNR1 OtB7. |
|---|---|
| Molecular Weight(MW) | 37 KDa |
| Cellular Localization | Membrane Single-pass type II membrane protein |
| SwissProt ID | Q8CJ91 |
|---|
Application
Fig1: Sample:Pancreas (Mouse) Lysate at 40 ug Primary: Anti-DC-SIGNR1/CD209b(ER1907-24)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 37kD
Application
Fig2: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-DC-SIGNR1/CD209b(ER1907-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD| Positive Control | Expressed in skin, spleen and lung, probably in a subset of dendritic cells. Detected in spleen extrafollicular paracortical areas including the red pulp and marginal zones, and at lower levels, in the follicular area. Detected in skin suprabasal areas adjacent to the epidermis and in epidermal cell layer. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
