Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-Deformed Epidermal Autoregulatory Factor 1 (ER1907-26) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD| Product Name | Deformed Epidermal Autoregulatory Factor 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DEAF1 Deformed epidermal autoregulatory factor 1 homolog Nuclear DEA1 related transcriptional regulator NUDR SPN Suppressin Zinc finger MYND domain containing protein 5 ZMYND5 DEAF1_HUMAN. |
| Product description | Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm. |
| Immunogen | KLH conjugated synthetic peptide derived from human Deformed Epidermal Autoregulatory Factor 1 211-310/565 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | MouseRatChickenDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | DEAF1 Deformed epidermal autoregulatory factor 1 homolog Nuclear DEA1 related transcriptional regulator NUDR SPN Suppressin Zinc finger MYND domain containing protein 5 ZMYND5 DEAF1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Isoform 1: Nucleus. Cytoplasm. Note=Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270%. ;Isoform 2: Secreted. Note=Secreted in some cell types. ;Isoform 3: Secreted. Note=Secreted in some cell types. ;Isoform 4: Cytoplasm. Nucleus. Note=When expressed alone, localizes mainly in the cytoplasm but, when expressed with isoform 1, nuclear localization is enhanced. |
| SwissProt ID | O75398 |
|---|
Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-Deformed Epidermal Autoregulatory Factor 1 (ER1907-26) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD| Positive Control | Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
