Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Deltex 1) Polyclonal Antibody, Unconjugated (ER1907-29) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Deltex 1 (ER1907-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD| Product Name | Deltex 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deltex protein 1 Deltex Deltex-1 Deltex1 Dtx1 FXI-T1 Fxit 1 Fxit1 mDTX1 DTX1_HUMAN. |
| Product description | Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1. |
| Immunogen | KLH conjugated synthetic peptide derived from human Deltex-1 351-450/620 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | Deltex protein 1 Deltex Deltex-1 Deltex1 Dtx1 FXI-T1 Fxit 1 Fxit1 mDTX1 DTX1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear. |
| SwissProt ID | Q86Y01 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Deltex 1) Polyclonal Antibody, Unconjugated (ER1907-29) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Deltex 1 (ER1907-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD| Positive Control | Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
