Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- DENND1B (ER1907-30) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 49 kD| Product Name | DENND1B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C1ORF18 C1orf218 Chromosome 1 open reading frame 218 Connecdenn 2 DEN1B_HUMAN DENN domain containing protein 1B DENN domain-containing protein 1B DENN MADD domain containing 1B DENND1B DKFZp547O0715 FAM31B Family with sequence similarity 31 member B FLJ20054 FLJ21179 FLJ33888 MGC103810 MGC27044 Protein FAM31B |
| Product description | Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactivates RAB35, then activates RAB35 for its subsequent function at endosomes. |
| Immunogen | KLH conjugated synthetic peptide derived from human DENND1B 241-350/426 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | C1ORF18 C1orf218 Chromosome 1 open reading frame 218 Connecdenn 2 DEN1B_HUMAN DENN domain containing protein 1B DENN domain-containing protein 1B DENN MADD domain containing 1B DENND1B DKFZp547O0715 FAM31B Family with sequence similarity 31 member B FLJ20054 FLJ21179 FLJ33888 MGC103810 MGC27044 Protein FAM31B |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle. |
| SwissProt ID | Q6P3S1 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- DENND1B (ER1907-30) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 49 kD| Positive Control | Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
