Application
Fig1: Protein:A549(human) lysates at 30ug; Primary: rabbit Anti-Dexras1 (ER1907-33) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:31 kD Observed band size: 31 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dexras1) Polyclonal Antibody, Unconjugated (ER1907-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dexras1) Polyclonal Antibody, Unconjugated (ER1907-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Dexras1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator of G protein signaling 1 Activator of G protein signaling Activator of G-protein signaling 1 AGS 1 AGS1 Dexamethasone induced ras related protein 1 Dexamethasone-induced ras protein 1 Dexamethasone-induced Ras-related protein 1 Dexras 1 MGC:26290 RAS dexamethasone induced 1 Ras protein, Dexamethasone-induced, 1 Ras related protein RASD 1 Rasd1 RASD1_HUMAN. |
| Product description | Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65. |
| Immunogen | KLH conjugated synthetic peptide derived from human Dexras1 21-120/281 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Activator of G protein signaling 1
Activator of G protein signaling
Activator of G-protein signaling 1
AGS 1
AGS1
Dexamethasone induced ras related protein 1
Dexamethasone-induced ras protein 1
Dexamethasone-induced Ras-related protein 1
Dexras 1
MGC:26290
RAS dexamethasone induced 1
Ras protein Dexamethasone-induced 1 Ras related protein RASD 1 Rasd1 RASD1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Cell membrane. Cytoplasm > perinuclear region. Nucleus. |
| SwissProt ID | Q9Y272 |
|---|
Application
Fig1: Protein:A549(human) lysates at 30ug; Primary: rabbit Anti-Dexras1 (ER1907-33) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:31 kD Observed band size: 31 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dexras1) Polyclonal Antibody, Unconjugated (ER1907-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dexras1) Polyclonal Antibody, Unconjugated (ER1907-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
