Application
Fig1: Sample:Hela Cell(Human)Lysate at 40 ug Primary: Anti-DGKA(ER1907-36)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83kD Observed band size: 83kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DGKA) Polyclonal Antibody, Unconjugated (ER1907-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | DGKA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 80 kDa diacylglycerol kinase DAG kinase alpha DAGK DAGK1 DGK alpha DGK-alpha dgkA DGKA_HUMAN Diacylglycerol kinase alpha Diacylglycerol kinase, alpha 80kDa Diglyceride kinase alpha MGC12821 MGC42356 OTTHUMP00000242836 OTTHUMP00000242955 OTTHUMP00000244046. |
| Product description | Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human DGKA 201-300/735 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 80 kDa diacylglycerol kinase
DAG kinase alpha
DAGK
DAGK1
DGK alpha
DGK-alpha
dgkA
DGKA_HUMAN
Diacylglycerol kinase alpha
Diacylglycerol kinase alpha 80kDa Diglyceride kinase alpha MGC12821 MGC42356 OTTHUMP00000242836 OTTHUMP00000242955 OTTHUMP00000244046. |
|---|---|
| Molecular Weight(MW) | 83 KDa |
| SwissProt ID | P23743 |
|---|
Application
Fig1: Sample:Hela Cell(Human)Lysate at 40 ug Primary: Anti-DGKA(ER1907-36)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83kD Observed band size: 83kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DGKA) Polyclonal Antibody, Unconjugated (ER1907-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Lymphocytes and oligodendroglial cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
