Application
Fig1: Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-DGKI(ER1907-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 117kD Observed band size: 117kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DGKI) Polyclonal Antibody, Unconjugated (ER1907-38) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | DGKI |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAG kinase iota DGK iota DGKiota DGKI_HUMAN Diacylglycerol kinase iota Diglyceride kinase iota. |
| Product description | This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. |
| Immunogen | KLH conjugated synthetic peptide derived from human DGKI 101-200/1065 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | DAG kinase iota DGK iota DGKiota DGKI_HUMAN Diacylglycerol kinase iota Diglyceride kinase iota. |
|---|---|
| Molecular Weight(MW) | 117 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O75912 |
|---|
Application
Fig1: Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-DGKI(ER1907-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 117kD Observed band size: 117kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DGKI) Polyclonal Antibody, Unconjugated (ER1907-38) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
