Application
Fig1: Sample: Kindey (Mouse) Lysate at 40 ug Primary: Anti- BPNT1 (bs-20136R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- DHCR7 (ER1907-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Product Name | DHCR7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 7 dehydrocholesterol reductase 7 DHC reductase Delta 7 dehydrocholesterol reductase Putative sterol reductase SR 2 Sterol Delta DHCR7_HUMAN. |
| Product description | Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC). 7-Dehydrocholesterol reductase, also known as DHCR7, is a protein that in humans is encoded by the DHCR7 gene. The protein encoded by this gene is an enzyme catalyzing the production of cholesterol from 7-Dehydrocholesterol using NADPH. The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the ultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development. |
| Immunogen | KLH conjugated synthetic peptide derived from human DHCR7 351-450/475 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | 7 dehydrocholesterol reductase
7 DHC reductase
Delta 7 dehydrocholesterol reductase Putative sterol reductase SR 2 Sterol Delta DHCR7_HUMAN. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9UBM7 |
|---|
Application
Fig1: Sample: Kindey (Mouse) Lysate at 40 ug Primary: Anti- BPNT1 (bs-20136R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- DHCR7 (ER1907-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Positive Control | Most abundant in adrenal gland, liver, testis, and brain. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
