Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (ER1907-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (ER1907-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (ER1907-45) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-DHX36 (ER1907-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kDApplication
Fig5: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-DHX36 (ER1907-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD| Product Name | DHX36 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX36 DEAH box protein 36 Dhx36 DHX36_HUMAN EC 3.6.1.- KIAA1488 MLE like protein 1 MLE-like protein 1 MLEL1 Probable ATP dependent RNA helicase DHX36 Probable ATP-dependent RNA helicase DHX36 RHAU RNA helicase associated with AU rich element ARE RNA helicase associated with AU-rich element ARE. |
| Product description | Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human DHX36 151-250/1008 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | DDX36 DEAH box protein 36 Dhx36 DHX36_HUMAN EC 3.6.1.- KIAA1488 MLE like protein 1 MLE-like protein 1 MLEL1 Probable ATP dependent RNA helicase DHX36 Probable ATP-dependent RNA helicase DHX36 RHAU RNA helicase associated with AU rich element ARE RNA helicase associated with AU-rich element ARE. |
|---|---|
| Molecular Weight(MW) | 115 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. |
| SwissProt ID | Q9H2U1 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (ER1907-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (ER1907-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (ER1907-45) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-DHX36 (ER1907-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD
Application
Fig5: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-DHX36 (ER1907-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD| Positive Control | Highly expressed in testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
