Application
Fig1: Sample: Adrenal glands (Mouse) Lysate at 40 ug Primary: Anti-DMT1 (ER1907-55) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (ER1907-55) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (ER1907-55) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (ER1907-55) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig5: Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-DMT1 antibody (ER1907-55) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig6: Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-DMT1 antibody (ER1907-55) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | DMT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCT 1 DCT1 Divalent metal transporter 1 DMT 1 DMT1 Natural resistance associated macrophage protein 2 NRAMP 2 NRAMP2 NRAM2_HUMAN. |
| Product description | Actin-bundling protein. May function in mitogen-activated protein kinase pathway. |
| Immunogen | KLH conjugated synthetic peptide derived from human DMT1 251-350/568 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | DCT 1 DCT1 Divalent metal transporter 1 DMT 1 DMT1 Natural resistance associated macrophage protein 2 NRAMP 2 NRAMP2 NRAM2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P49281 |
|---|
Application
Fig1: Sample: Adrenal glands (Mouse) Lysate at 40 ug Primary: Anti-DMT1 (ER1907-55) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (ER1907-55) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (ER1907-55) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (ER1907-55) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig5: Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-DMT1 antibody (ER1907-55) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig6: Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-DMT1 antibody (ER1907-55) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Heart, brain, lung, skeletal muscle, and kidney. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
