Application
Fig1: Sample:HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-DNA polymerase mu (ER1907-58)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 60kDApplication
Fig2: Sample: Raji Cell(Human)Lysate at 30 ug Primary: Anti-DNA polymerase mu (ER1907-58)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 60kD| Product Name | DNA polymerase mu |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA Polymerase Mu DNA-directed DNA polymerase mu DNA-directed DNA/RNA polymerase mu DPOLM_HUMAN FLJ35482 Pol iota Pol Mu Polm POLM protein Polymerase (DNA directed) mu Polymerase DNA directed mu Tdt N Tdt-N TdtN Terminal transferase. |
| Product description | Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. |
| Immunogen | KLH conjugated synthetic peptide derived from human DNA polymerase mu 261-360/494 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatHorse |
| Concentration | 1mg/ml |
| Alternative Names | DNA Polymerase Mu DNA-directed DNA polymerase mu DNA-directed DNA/RNA polymerase mu DPOLM_HUMAN FLJ35482 Pol iota Pol Mu Polm POLM protein Polymerase (DNA directed) mu Polymerase DNA directed mu Tdt N Tdt-N TdtN Terminal transferase. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9NP87 |
|---|
Application
Fig1: Sample:HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-DNA polymerase mu (ER1907-58)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 60kD
Application
Fig2: Sample: Raji Cell(Human)Lysate at 30 ug Primary: Anti-DNA polymerase mu (ER1907-58)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 60kD| Positive Control | Expressed in a number of tissues. Abundant in thymus. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
