Application
Fig1: Protein: K562(human) lysates at 30ug; Primary: rabbit Anti-DNA primase (ER1907-59) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:60 kD Observed band size:60 kDApplication
Fig2: Sample: bone (mouse) Lysate at 40 ug testis (Mouse) Lysate at 40 ug Primary: Anti-DNA primase (ER1907-59) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 63 kD| Product Name | DNA Primase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ422B11.1.1 DNA primase 58kDa subunit DNA primase large subunit DNA primase p58 DNA primase subunit p58 p58 Polypeptide 2A p58 PRIM2 PRIM2A PRI2_HUMAN Primase DNA polypeptide 2 (58kDa) Primase polpeptide 2A (58kD) Primase polpeptide 2A 58kDa. |
| Product description | DNA primase synthesizes small ribonucleotide primers for the initiation of DNA replication. The eukaryotic primase is a heterodimer of a small and a large subunit, p49 and p58 respectively. These two subunits purify as a complex tightly bound to DNA polymerase alpha. This tight association implicates polymerase alpha as the lagging-strand DNA polymerase in replication. Uniquely, primases are able to synthesize nucleotides de novo on a template by the formation of an initial dinucleotide. DNA primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. The human primase subunits are 90% identical in amino acid sequence to the mouse homologs. |
| Immunogen | KLH conjugated synthetic peptide derived from human DNA Primase 51-150/509 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | dJ422B11.1.1 DNA primase 58kDa subunit DNA primase large subunit DNA primase p58 DNA primase subunit p58 p58 Polypeptide 2A p58 PRIM2 PRIM2A PRI2_HUMAN Primase DNA polypeptide 2 (58kDa) Primase polpeptide 2A (58kD) Primase polpeptide 2A 58kDa. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| SwissProt ID | P49643 |
|---|
Application
Fig1: Protein: K562(human) lysates at 30ug; Primary: rabbit Anti-DNA primase (ER1907-59) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:60 kD Observed band size:60 kD
Application
Fig2: Sample: bone (mouse) Lysate at 40 ug testis (Mouse) Lysate at 40 ug Primary: Anti-DNA primase (ER1907-59) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 63 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
