Application
Fig1: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min; Incubation: Anti-DNase gamma Polyclonal Antibody, Unconjugated(ER1907-63) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-DNase gamma (bs-7563R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 36 kD Observed band size: 32/36 kD| Product Name | DNase gamma |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deoxyribonuclease gamma Deoxyribonuclease I like 3 Deoxyribonuclease I like III Deoxyribonuclease I-like 3 DHP2 DNAS1L3 DNase gamma DNase I homolog protein 2 DNase I homolog protein DHP2 DNase I like 3 DNase I-like 3 DNASE1L3 DNSL3_HUMAN Liver and spleen DNase LS DNase LS-DNase LSD. |
| Product description | Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units. |
| Immunogen | KLH conjugated synthetic peptide derived from human DNase gamma 151-250/305 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Deoxyribonuclease gamma Deoxyribonuclease I like 3 Deoxyribonuclease I like III Deoxyribonuclease I-like 3 DHP2 DNAS1L3 DNase gamma DNase I homolog protein 2 DNase I homolog protein DHP2 DNase I like 3 DNase I-like 3 DNASE1L3 DNSL3_HUMAN Liver and spleen DNase LS DNase LS-DNase LSD. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13609 |
|---|
Application
Fig1: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min; Incubation: Anti-DNase gamma Polyclonal Antibody, Unconjugated(ER1907-63) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-DNase gamma (bs-7563R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 36 kD Observed band size: 32/36 kD| Positive Control | Liver and spleen. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
