Application
Fig1: Tissue/cell: Human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DNCLI1 Polyclonal Antibody, Unconjugated(ER1907-65) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- DNCLI1 (ER1907-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD| Product Name | DNCLI1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytoplasmic dynein 1 light intermediate chain 1 cytosolic DC1L1_HUMAN DLC-A DNCLI1 dync1li1 Dynein cytoplasmic 1 light intermediate chain 1 Dynein cytoplasmic light intermediate polypeptide 1 Dynein light chain A Dynein light intermediate chain 1 Dynein light intermediate chain 1 cytosolic FLJ10219 LIC1. |
| Product description | Cytomegalovirus (CMV) is a member of the Herpes virus family. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid which is embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope that is decorated with virus-specific glycoprotein spikes. The viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. Cytomegalovirus strain AD169 is a laboratory-adapted strain and appears to lack a 15kb region of the 200kb genome that is present in clinical isolates. This region contains 19 open reading frames whose functions have yet to be elucidated. AD169 is also unique in that it is unable to enter latency and nearly always assumes lytic growth upon infection. |
| Immunogen | KLH conjugated synthetic peptide derived from human DNCLI1 451-550/523 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChicken |
| Concentration | 1mg/ml |
| Alternative Names | Cytoplasmic dynein 1 light intermediate chain 1 cytosolic DC1L1_HUMAN DLC-A DNCLI1 dync1li1 Dynein cytoplasmic 1 light intermediate chain 1 Dynein cytoplasmic light intermediate polypeptide 1 Dynein light chain A Dynein light intermediate chain 1 Dynein light intermediate chain 1 cytosolic FLJ10219 LIC1. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| SwissProt ID | Q9Y6G9 |
|---|
Application
Fig1: Tissue/cell: Human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DNCLI1 Polyclonal Antibody, Unconjugated(ER1907-65) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- DNCLI1 (ER1907-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
