Application
Fig1: Sample: H9C2 Cell (Rat) Lysate at 40 ug Primary: Anti-DOK7 (ER1907-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 63 kD| Product Name | DOK7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Docking protein 7 DOK 7 DOK7 DOK7_HUMAN Downstream of tyrosine kinase 7 Protein Dok-7. |
| Product description | Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK. |
| Immunogen | KLH conjugated synthetic peptide derived from human DOK7 21-120/504 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Docking protein 7 DOK 7 DOK7 DOK7_HUMAN Downstream of tyrosine kinase 7 Protein Dok-7. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Cell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions. |
| SwissProt ID | Q18PE1 |
|---|
Application
Fig1: Sample: H9C2 Cell (Rat) Lysate at 40 ug Primary: Anti-DOK7 (ER1907-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 63 kD| Positive Control | Preferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
