Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-DOLPP1 (ER1907-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DOLPP1) Polyclonal Antibody, Unconjugated (ER1907-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | DOLPP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 0610011H20Rik AB030189 DOPP1_HUMAN Dolichyldiphosphatase 1 Linked to Surfeit genes in Fugu rubripes 2 LSFR2 OTTHUMP00000022326 OTTHUMP00000196902. |
| Product description | DOLPP1 is a multi-pass membrane protein(By similarity). It belongs to the dolichyldiphosphatase family. It is required for efficient N-glycosylation and is necessary for maintaining optimal levels of dolichol-linked oligosaccharides. DOLPP1 hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. It does not act on phosphatidate |
| Immunogen | KLH conjugated synthetic peptide derived from human DOLPP1 151-238/238 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | 0610011H20Rik AB030189 DOPP1_HUMAN Dolichyldiphosphatase 1 Linked to Surfeit genes in Fugu rubripes 2 LSFR2 OTTHUMP00000022326 OTTHUMP00000196902. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). |
| SwissProt ID | Q86YN1 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-DOLPP1 (ER1907-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DOLPP1) Polyclonal Antibody, Unconjugated (ER1907-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
