Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (ER1907-71) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti-Dopamine D3 receptor (ER1907-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kDApplication
Fig3: Sample: HepG2 Cell Lysate at 30 ug U251 Cell Lysate at 30 ug Primary: Anti-Dopamine D3 receptor (ER1907-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD| Product Name | Dopamine D3 receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DRD3 3dopamine receptor D Dopamine receptor D3 DRD3 Dopamine receptor 3 ETM1 FET1 D(3) dopamine receptor DRD3 DRD3_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse DRD3 352-446/446 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DRD3 3dopamine receptor D Dopamine receptor D3 DRD3 Dopamine receptor 3 ETM1 FET1 D(3) dopamine receptor DRD3 DRD3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. |
| SwissProt ID | P30728 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (ER1907-71) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti-Dopamine D3 receptor (ER1907-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD
Application
Fig3: Sample: HepG2 Cell Lysate at 30 ug U251 Cell Lysate at 30 ug Primary: Anti-Dopamine D3 receptor (ER1907-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD| Positive Control | Brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
