Application
Fig1: Sample:
Lane1: Brain(Mouse) Lysate at 30 ug
Lane2: Liver(Mouse) Lysate at 30 ug
Primary: Anti-DRD4(ER1907-72) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 41kD
Observed band size : 41kD
Application
Fig2: Sample:
A549(Human) Cell Lysate at 30 ug
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti- Dopamine D4 receptor (ER1907-72) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 53 kD
Application
Fig3: Sample:
Hela(Human) Cell Lysate at 30 ug
Urinary bladder (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D4 receptor (ER1907-72) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41/51 kD
Observed band size: 51 kD