Application
Fig1: Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- DRD5 antibody(ER1907-73), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (ER1907-73, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1907-73 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti- Dopamine D5 receptor (ER1907-73)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53kD Observed band size: 53kD| Product Name | Dopamine D5 receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D(1B) dopamine receptor D(5) dopamine receptor D1 beta dopamine receptor D1B dopamine receptor D1beta dopamine receptor D5 dopamine receptor DBDR Dopamine receptor D1B DRD 1B DRD 5 DRD1B DRD1L2 DRD5 MGC10601 DRD5_HUMAN DRD5_RAT. |
| Immunogen | KLH conjugated synthetic peptide derived from rat DRD5 251-350/475 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | D(1B) dopamine receptor D(5) dopamine receptor D1 beta dopamine receptor D1B dopamine receptor D1beta dopamine receptor D5 dopamine receptor DBDR Dopamine receptor D1B DRD 1B DRD 5 DRD1B DRD1L2 DRD5 MGC10601 DRD5_HUMAN DRD5_RAT. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P25115 |
|---|
Application
Fig1: Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- DRD5 antibody(ER1907-73), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (ER1907-73, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1907-73 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti- Dopamine D5 receptor (ER1907-73)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53kD Observed band size: 53kD| Positive Control | Neuron-specific, localized primarily within limbic regions of the brain. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
