Application
Fig1: Positive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: ER1907-74; Dilution: 12μg in 100 μl 1X PBS containing 0.5% BSAApplication
Fig2: Positive control: RSC96 Isotype Control Antibody: rabbit IgG-PE/CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.Application
Fig3: Blank control: U937 (blue). Primary Antibody:Rabbit Anti-Dopamine Transporter antibody(ER1907-74), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1907-74, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (ER1907-74) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig5: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Dopamine Transporter (ER1907-74) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kDApplication
Fig6: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (ER1907-74) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Dopamine Transporter |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DA transporter DAT 1 DAT DAT1 Dopamine transporter SC6A3_HUMAN SLC6A3 Sodium dependent dopamine transporter Sodium-dependent dopamine transporter Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 Solute carrier family 6 member 3 Variable number tandem repeat (VNTR) dopamine transporter ADAT 1 Adenosine deaminase tRNA specific 1 HADAT1 tRNA specific adenosine deaminase 1. |
| Product description | Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. |
| Immunogen | KLH conjugated synthetic peptide derived from human DAT1 251-350/620 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:0.2μg /test: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | DA transporter
DAT 1
DAT
DAT1
Dopamine transporter
SC6A3_HUMAN
SLC6A3
Sodium dependent dopamine transporter
Sodium-dependent dopamine transporter
Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 Solute carrier family 6 member 3 Variable number tandem repeat (VNTR) dopamine transporter ADAT 1 Adenosine deaminase tRNA specific 1 HADAT1 tRNA specific adenosine deaminase 1. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | Q01959 |
|---|
Application
Fig1: Positive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: ER1907-74; Dilution: 12μg in 100 μl 1X PBS containing 0.5% BSA
Application
Fig2: Positive control: RSC96 Isotype Control Antibody: rabbit IgG-PE/CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.
Application
Fig3: Blank control: U937 (blue). Primary Antibody:Rabbit Anti-Dopamine Transporter antibody(ER1907-74), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1907-74, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (ER1907-74) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig5: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Dopamine Transporter (ER1907-74) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD
Application
Fig6: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (ER1907-74) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:0.2μg /test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
