Application
Fig1: Sample: Hela Cell (Human) Lysate at 30 ug MCF-7Cell (Human) Lysate at 30 ug Primary: Anti-DPH2 (ER1907-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kDApplication
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-DPH2 (ER1907-76) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 60 kD| Product Name | DPH2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Diphthamide biosynthesis like protein 2 Diphthamide biosynthesis protein 2 Diphthamide biosynthesis protein 2 homolog like 2 Diphthamide biosynthesis protein 2 homolog-like 2 Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae DPH 2 DPH2 DPH2 homolog DPH2 homolog S. cerevisiae DPH2 like 2 DPH2 like 2 S. cerevisiae DPH2-like 2 DPH2_HUMAN DPH2L2 HsDph2 OTTHUMP00000010007 Protein DPH2 homolog. |
| Product description | Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human DPH2 401-489/489 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Diphthamide biosynthesis like protein 2 Diphthamide biosynthesis protein 2 Diphthamide biosynthesis protein 2 homolog like 2 Diphthamide biosynthesis protein 2 homolog-like 2 Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae DPH 2 DPH2 DPH2 homolog DPH2 homolog S. cerevisiae DPH2 like 2 DPH2 like 2 S. cerevisiae DPH2-like 2 DPH2_HUMAN DPH2L2 HsDph2 OTTHUMP00000010007 Protein DPH2 homolog. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| SwissProt ID | Q9BQC3 |
|---|
Application
Fig1: Sample: Hela Cell (Human) Lysate at 30 ug MCF-7Cell (Human) Lysate at 30 ug Primary: Anti-DPH2 (ER1907-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD
Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-DPH2 (ER1907-76) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 60 kD| Positive Control | Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
