Application
Fig1: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAILR1 Polyclonal Antibody, Unconjugated(ER1907-78) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
g2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DR4 Polyclonal Antibody, Unconjugated(ER1907-78) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: K562(Human) Cell Lysate at 40 ug Primary: Anti- DR4 (ER1907-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kDApplication
Fig4: Sample: K562(Human) Cell Lysate at 40 ug Primary: Anti- DR4 (ER1907-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD| Product Name | DR4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apo 2 Apo2 CD 261 CD261 CD261 antigen Cytotoxic ligand TRAIL receptor Cytotoxic TRAIL receptor Death receptor 4 DR 4 DR4 MGC9365 NF related apoptosis-inducing ligand receptor 1 T10A TNF related apoptosis inducing ligand receptor 1 TNFRSF10A TRAIL R1 TRAIL receptor 1 TRAILR 1 TRAILR1 Tumor necrosis factor receptor superfamily member 10a Tumor necrosis factor receptor superfamily member 10A precursor TR10A_HUMAN. |
| Product description | Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. |
| Immunogen | KLH conjugated synthetic peptide derived from human DR4 401-468/468 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | Apo 2 Apo2 CD 261 CD261 CD261 antigen Cytotoxic ligand TRAIL receptor Cytotoxic TRAIL receptor Death receptor 4 DR 4 DR4 MGC9365 NF related apoptosis-inducing ligand receptor 1 T10A TNF related apoptosis inducing ligand receptor 1 TNFRSF10A TRAIL R1 TRAIL receptor 1 TRAILR 1 TRAILR1 Tumor necrosis factor receptor superfamily member 10a Tumor necrosis factor receptor superfamily member 10A precursor TR10A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | O00220 |
|---|
Application
Fig1: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAILR1 Polyclonal Antibody, Unconjugated(ER1907-78) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
g2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DR4 Polyclonal Antibody, Unconjugated(ER1907-78) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: K562(Human) Cell Lysate at 40 ug Primary: Anti- DR4 (ER1907-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD
Application
Fig4: Sample: K562(Human) Cell Lysate at 40 ug Primary: Anti- DR4 (ER1907-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD| Positive Control | Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
