Application
Fig1: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated(ER1907-81) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: EC-9706(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (ER1907-81) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kD Observed band size: 129 kDApplication
Fig3: Sample: LOVO(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (ER1907-81) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kD Observed band size: 67/129 kD| Product Name | DSPP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dentin phosphophoryn Dentin phosphoprotein dentin phosphoryn Dentin sialophosphoprotein Dentin sialophosphoprotein precursor Dentin sialoprotein dentinogenesis imperfecta 1 DFNA39 DGI1 DMP3 DPP DSP DSPP_HUMAN DTDP2. |
| Product description | DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals. |
| Immunogen | KLH conjugated synthetic peptide derived from human Dentin sialoprotein 31-130/1301 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Dentin phosphophoryn Dentin phosphoprotein dentin phosphoryn Dentin sialophosphoprotein Dentin sialophosphoprotein precursor Dentin sialoprotein dentinogenesis imperfecta 1 DFNA39 DGI1 DMP3 DPP DSP DSPP_HUMAN DTDP2. |
|---|---|
| Molecular Weight(MW) | 47/129 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | Q9NZW4 |
|---|
Application
Fig1: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated(ER1907-81) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: EC-9706(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (ER1907-81) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kD Observed band size: 129 kD
Application
Fig3: Sample: LOVO(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (ER1907-81) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kD Observed band size: 67/129 kD| Positive Control | Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
