Application
Fig1: Sample: TT(Human) Cell Lysate at 30 ug Testis (Rat) Lysate at 40 ug Primary: Anti- DUOX1 (ER1907-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 175 kD Observed band size: 200 kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- DUOX1 (ER1907-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 175 kD Observed band size: 200 kD| Product Name | DUOX1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual oxidase 1 Dual oxidase 1 precursor DUOX1 EC 1.11.1 EC 1.6.3.1 Flavoprotein NADPH oxidase Large NOX 1 LNOX1 Long NOX 1 MGC138840 MGC138841 NADPH thyroid oxidase 1 Nicotinamide adenine dinucleotide phosphate oxidase NOXEF1 THOX1 Thyroid oxidase 1 DUOX1_HUMAN. |
| Product description | The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene (from EntrezGene). |
| Immunogen | KLH conjugated synthetic peptide derived from human DUOX1 1-100/1551 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Dual oxidase 1 Dual oxidase 1 precursor DUOX1 EC 1.11.1 EC 1.6.3.1 Flavoprotein NADPH oxidase Large NOX 1 LNOX1 Long NOX 1 MGC138840 MGC138841 NADPH thyroid oxidase 1 Nicotinamide adenine dinucleotide phosphate oxidase NOXEF1 THOX1 Thyroid oxidase 1 DUOX1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 175 KDa |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. Note=Localizes to the apical membrane of epithelial cells. |
| SwissProt ID | Q9NRD9 |
|---|
Application
Fig1: Sample: TT(Human) Cell Lysate at 30 ug Testis (Rat) Lysate at 40 ug Primary: Anti- DUOX1 (ER1907-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 175 kD Observed band size: 200 kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- DUOX1 (ER1907-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 175 kD Observed band size: 200 kD| Positive Control | Expressed in thyrocytes and tracheal surface epithelial cells (at protein level). Expressed in thyroid, trachea, bronchium, and to a lower extent, in placenta, testis, prostate, pancreas and heart. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
