Application
Fig1: Sample: RAW264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-DUSP4 (ER1907-84) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kDApplication
Fig2: Blank control:A431. Primary Antibody (green line): Rabbit Anti-DUSP4 antibody (ER1907-84) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | DUSP4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen activated protein kinase phosphatase 2 Dual specificity phosphatase 4 Dual specificity protein phosphatase 4 Dual specificity protein phosphatase hVH2 DUSP 4 HVH 2 HVH2 MAP kinase phosphatase 2 Mitogen activated protein kinase phosphatase 2 MKP 2 MKP-2 MKP2 Serine/threonine specific protein phosphatase TYP VH 2 VH1 homologous phosphatase 2 VH2. |
| Immunogen | KLH conjugated synthetic peptide derived from human MKP-2 301-394/394 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Mitogen activated protein kinase phosphatase 2 Dual specificity phosphatase 4 Dual specificity protein phosphatase 4 Dual specificity protein phosphatase hVH2 DUSP 4 HVH 2 HVH2 MAP kinase phosphatase 2 Mitogen activated protein kinase phosphatase 2 MKP 2 MKP-2 MKP2 Serine/threonine specific protein phosphatase TYP VH 2 VH1 homologous phosphatase 2 VH2. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13115 |
|---|
Application
Fig1: Sample: RAW264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-DUSP4 (ER1907-84) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD
Application
Fig2: Blank control:A431. Primary Antibody (green line): Rabbit Anti-DUSP4 antibody (ER1907-84) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-2000: FC:1ug/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
