Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- E2F3 (ER1907-86) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD| Product Name | E2F3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686C18211 E2F 3 E2F transcription factor 3 KIAA0075 MGC104598 Transcription factor E2F 3 E2F3_HUMAN Transcription factor E2F3 E2F-3. |
| Product description | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. |
| Immunogen | KLH conjugated synthetic peptide derived from human E2F3 381-465/465 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp686C18211 E2F 3 E2F transcription factor 3 KIAA0075 MGC104598 Transcription factor E2F 3 E2F3_HUMAN Transcription factor E2F3 E2F-3. |
|---|---|
| Molecular Weight(MW) | 49 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O00716 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- E2F3 (ER1907-86) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
