Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- E3 ubiquitin ligase (ER1907-88) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 40 kD Observed band size: 40 kD| Product Name | E3 ubiquitin ligase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C1orf166 E3 ubiquitin ligase E3 ubiquitin protein ligase MUL1 FLJ12875 GIDE Growth inhibition and death E3 ligase MAPL Mitochondrial anchored protein ligase Mitochondrial ubiquitin ligase activator of NFKB 1 MUL1 MULAN Putative NF kappa B activating protein 266 RING finger protein 218 RNF218 RP23-25C1.10-002. |
| Product description | Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Immunogen | KLH conjugated synthetic peptide derived from human E3 ubiquitin ligase/RNF218 51-150/352 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | C1orf166 E3 ubiquitin ligase E3 ubiquitin protein ligase MUL1 FLJ12875 GIDE Growth inhibition and death E3 ligase MAPL Mitochondrial anchored protein ligase Mitochondrial ubiquitin ligase activator of NFKB 1 MUL1 MULAN Putative NF kappa B activating protein 266 RING finger protein 218 RNF218 RP23-25C1.10-002. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein. Peroxisome. Note: Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome. |
| SwissProt ID | Q969V5 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- E3 ubiquitin ligase (ER1907-88) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 40 kD Observed band size: 40 kD| Positive Control | Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
