Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EB2) Polyclonal Antibody, Unconjugated (ER1907-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- EB2 (ER1907-89) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD| Product Name | EB2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC binding protein EB2 APC-binding protein EB2 EB1 EB2 End binding protein 2 End-binding protein 2 H.sapiens mRNA for novel T cell activation protein MAPRE2 MARE2_HUMAN Microtubule associated protein RP/EB family member 2 Microtubule-associated protein RP/EB family member 2 T cell activation protein EB1 family. |
| Product description | May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. |
| Immunogen | KLH conjugated synthetic peptide derived from human End binding protein 2 101-200/327 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | APC binding protein EB2 APC-binding protein EB2 EB1 EB2 End binding protein 2 End-binding protein 2 H.sapiens mRNA for novel T cell activation protein MAPRE2 MARE2_HUMAN Microtubule associated protein RP/EB family member 2 Microtubule-associated protein RP/EB family member 2 T cell activation protein EB1 family. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| SwissProt ID | Q15555 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EB2) Polyclonal Antibody, Unconjugated (ER1907-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- EB2 (ER1907-89) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD| Positive Control | Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
