Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (ER1907-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kDApplication
Fig2: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (ER1907-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kDApplication
Fig3: Sample: Skin(Mouse) Lysate at 40 ug Primary: Anti-ECM1 (ER1907-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kDApplication
Fig4: Sample: MDA-MB-231(Mouse) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (ER1907-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 65 kD| Product Name | ECM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Secretory Component Glycoprotein ECM 1 Ecm1 ECM1_HUMAN Extracellular matrix protein 1 Secretory component p85. |
| Product description | Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human ECM1 488-567/567 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | Secretory Component Glycoprotein ECM 1 Ecm1 ECM1_HUMAN Extracellular matrix protein 1 Secretory component p85. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | Q61508 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (ER1907-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kD
Application
Fig2: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (ER1907-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kD
Application
Fig3: Sample: Skin(Mouse) Lysate at 40 ug Primary: Anti-ECM1 (ER1907-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kD
Application
Fig4: Sample: MDA-MB-231(Mouse) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (ER1907-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 65 kD| Positive Control | Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
