Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-ECT2 (ER1907-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 108 kD| Product Name | ECT2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ECT 2 ECT2 ECT-2 ECT2 protein Epithelial cell transforming sequence 2 Epithelial cell transforming sequence 2 oncogene Epithelial cell transforming sequence 2 oncogene protein FLJ10461 MGC138291 Protein ECT2 ECT2_HUMAN Epithelial cell-transforming sequence 2 oncogene. |
| Product description | Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits Phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. |
| Immunogen | KLH conjugated synthetic peptide derived from human ECT2 781-883/914 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ECT 2 ECT2 ECT-2 ECT2 protein Epithelial cell transforming sequence 2 Epithelial cell transforming sequence 2 oncogene Epithelial cell transforming sequence 2 oncogene protein FLJ10461 MGC138291 Protein ECT2 ECT2_HUMAN Epithelial cell-transforming sequence 2 oncogene. |
|---|---|
| Molecular Weight(MW) | 100 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells. |
| SwissProt ID | Q9H8V3 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-ECT2 (ER1907-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 108 kD| Positive Control | Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. [INDUCTION] Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
