Application
Fig1: Sample: B16 Cell (Mouse) Lysate at 40 ug DU145 Cell (Human) Lysate at 40 ug Primary: Anti-EGFR5 (ER1907-97) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 53 kDApplication
Fig2: Blank control(blue):293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-EGFR5 antibody(ER1907-97), Dilution: 1μg in 100μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | EGFR5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C type lectin domain family 14, member A C14orf27 CEG1 ClECT and EGF like domain containing protein EGFR5 EGFR-5 EGFR 5 Epidermal growth factor receptor 5 CLC14_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A 221-320/490 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | C type lectin domain family 14 member A C14orf27 CEG1 ClECT and EGF like domain containing protein EGFR5 EGFR-5 EGFR 5 Epidermal growth factor receptor 5 CLC14_HUMAN. |
|---|---|
| Molecular Weight(MW) | 49 KDa |
| Cellular Localization | Membrane; Single pass type I membrane protein. |
| SwissProt ID | Q86T13 |
|---|
Application
Fig1: Sample: B16 Cell (Mouse) Lysate at 40 ug DU145 Cell (Human) Lysate at 40 ug Primary: Anti-EGFR5 (ER1907-97) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 53 kD
Application
Fig2: Blank control(blue):293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-EGFR5 antibody(ER1907-97), Dilution: 1μg in 100μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
