Application
Fig1: The figure annotation: The blue histogram is unstained cells.The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (ER1908-02)plus secondary antibody. Controls: Positive control: A549 cells .Isotype control: Cell lines treated with rabbit IgG (bs-0295P)instead of the primary antibody to confirm that primary antibody binding is specific.Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC) to confirm no background signal produced from secondary antibody alone. 1ug in 100uL 1 X PBS containing 0.5% BSA.Application
Fig2: Sample: Lung (Mouse) Lysate at 30 ug Primary: Anti- alpha Elastin/Tropoelastin (ER1908-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 70 kD Observed band size: 68 kD| Product Name | Elastin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | alpha Elastin/Tropoelastin Elastin isoform a ELN ELN_HUMAN elastin isoform m precursor FLJ38671 FLJ43523 Supravalvular aortic stenosis Tropoelastin Williams Beuren syndrome Williams syndrome region ADCL1 SVAS WBS WS. |
| Product description | Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. |
| Immunogen | KLH conjugated synthetic peptide derived from human Elastin 681-786/786 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | alpha Elastin/Tropoelastin Elastin isoform a ELN ELN_HUMAN elastin isoform m precursor FLJ38671 FLJ43523 Supravalvular aortic stenosis Tropoelastin Williams Beuren syndrome Williams syndrome region ADCL1 SVAS WBS WS. |
|---|---|
| Molecular Weight(MW) | 70 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers. |
| SwissProt ID | P15502 |
|---|
Application
Fig1: The figure annotation: The blue histogram is unstained cells.The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (ER1908-02)plus secondary antibody. Controls: Positive control: A549 cells .Isotype control: Cell lines treated with rabbit IgG (bs-0295P)instead of the primary antibody to confirm that primary antibody binding is specific.Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC) to confirm no background signal produced from secondary antibody alone. 1ug in 100uL 1 X PBS containing 0.5% BSA.
Application
Fig2: Sample: Lung (Mouse) Lysate at 30 ug Primary: Anti- alpha Elastin/Tropoelastin (ER1908-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 70 kD Observed band size: 68 kD| Positive Control | Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
