Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-EMP-1 (ER1908-05) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 19 kDApplication
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-EMP-1 antibody (ER1908-05) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | EMP-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CL-20 EMP 1 EMP1 EMP1_HUMAN Epithelial membrane protein 1 Protein B4B TMP Tumor-associated membrane protein. |
| Product description | EMP1 belongs to the peripheral myelin protein 22-kDa gene family that encompasses proteins with tetra-spanning membrane domains, and has a high degree of domain structure homology with EMP2 and EMP3. Although EMP1 is reportedly downregulated in the cancer region as compared with the adjacent normal epithelium at the primary tumor site, this molecule has also been proposed as a marker of resistance to cancer therapies and related to poor prognosis. With respect to clinical implications, development of an inhibitory antibody to specifically target EMP1 to infer the potential of this molecule may be challenging because of the high expression of EMP1 in pro-metastatic cancers. The intracellular loop region of EMP1 also represents a candidate target to inhibit cancer metastasis, because EMP1-induced signal transduction is triggered by the complex formation of EMP1 and copine-III. EMP-1 may act as oncogene or suppressor gene in different cancers. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Emp1 101-160/160 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CL-20 EMP 1 EMP1 EMP1_HUMAN Epithelial membrane protein 1 Protein B4B TMP Tumor-associated membrane protein. |
|---|---|
| Molecular Weight(MW) | 17 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | P47801 |
|---|
Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-EMP-1 (ER1908-05) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 19 kD
Application
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-EMP-1 antibody (ER1908-05) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
