Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ENO3 Polyclonal Antibody, Unconjugated(ER1908-12) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ENO3 (ER1908-12) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 49 kDApplication
Fig3: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ENO3 antibody (ER1908-12) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | ENO3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2 phospho D glycerate hydrol yase 2 phospho D glycerate hydrolyase Beta enolase ENO3 Enolase 3 (beta muscle) Enolase 3 Enolase3 GSD13 MSE Muscle specific enolase Skeletal muscle enolase ENOB_HUMAN. |
| Product description | Appears to have a function in striated muscle development and regeneration. |
| Immunogen | KLH conjugated synthetic peptide derived from human ENO3 341-434/434 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:2ug/Test': |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | 2 phospho D glycerate hydrol yase 2 phospho D glycerate hydrolyase Beta enolase ENO3 Enolase 3 (beta muscle) Enolase 3 Enolase3 GSD13 MSE Muscle specific enolase Skeletal muscle enolase ENOB_HUMAN. |
|---|---|
| Molecular Weight(MW) | 47 KDa |
| Cellular Localization | Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity). |
| SwissProt ID | P13929 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ENO3 Polyclonal Antibody, Unconjugated(ER1908-12) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ENO3 (ER1908-12) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 49 kD
Application
Fig3: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ENO3 antibody (ER1908-12) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:2ug/Test': |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
