Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Pancreas (Mouse) Lysate at 40 ug Primary: Anti-ENPP1 (ER1908-13) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 100 kD Observed band size: 100 kD| Product Name | ENPP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkaline phosphodiesterase 1 ARHR2 E-NPP 1 Ectonucleotide pyrophosphatase/phosphodiesterase 1 Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ENPP1_HUMAN Ly 41 antigen M6S1 Membrane component chromosome 6 surface marker 1 NPP1 NPPase NPPS Nucleotide pyrophosphatase PC-1 PCA1 PDNP1 Phosphodiesterase I/nucleotide pyrophosphatase 1 Plasma cell membrane glycoprotein 1 Plasma-cell membrane glycoprotein PC-1. |
| Product description | Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. |
| Immunogen | KLH conjugated synthetic peptide derived from human ENPP1 41-140/925 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Alkaline phosphodiesterase 1 ARHR2 E-NPP 1 Ectonucleotide pyrophosphatase/phosphodiesterase 1 Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ENPP1_HUMAN Ly 41 antigen M6S1 Membrane component chromosome 6 surface marker 1 NPP1 NPPase NPPS Nucleotide pyrophosphatase PC-1 PCA1 PDNP1 Phosphodiesterase I/nucleotide pyrophosphatase 1 Plasma cell membrane glycoprotein 1 Plasma-cell membrane glycoprotein PC-1. |
|---|---|
| Molecular Weight(MW) | 100 KDa |
| Cellular Localization | Membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Note=Targeted to the basolateral membrane in polarized epithelial cells and in hepatocytes, and to matrix vesicles in osteoblasts. In bile duct cells and cancer cells, located to the apical cytoplasmic side. |
| SwissProt ID | P22413 |
|---|
Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Pancreas (Mouse) Lysate at 40 ug Primary: Anti-ENPP1 (ER1908-13) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 100 kD Observed band size: 100 kD| Positive Control | Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
