Application
Fig1: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-Anei-ENPP2 (ER1908-14) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kD Observed band size: 115 kDApplication
Fig2: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Anei-ATX/Autotaxin Polyclonal Antibody, Unconjugated(ER1908-14) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | ENPP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Autotaxin Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ATX ATX X ATX-X Autotaxin E NPP 2 ENPP2 Ectonucleotide pyrophosphatase/phosphodiesterase 2 Extracellular lysophospholipase D FLJ26803 LysoPLD NPP2 PD IALPHA PD-IALPHA PDNP2 Phosphodiesterase I alpha Phosphodiesterase I/nucleotide pyrophosphatase 2 Plasma lysophospholipase D ENPP2_HUMAN. |
| Product description | Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. |
| Immunogen | KLH conjugated synthetic peptide derived from human ENPP2 601-700/915 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Autotaxin Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ATX ATX X ATX-X Autotaxin E NPP 2 ENPP2 Ectonucleotide pyrophosphatase/phosphodiesterase 2 Extracellular lysophospholipase D FLJ26803 LysoPLD NPP2 PD IALPHA PD-IALPHA PDNP2 Phosphodiesterase I alpha Phosphodiesterase I/nucleotide pyrophosphatase 2 Plasma lysophospholipase D ENPP2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 95 KDa |
| Cellular Localization | Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells. |
| SwissProt ID | Q13822 |
|---|
Application
Fig1: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-Anei-ENPP2 (ER1908-14) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kD Observed band size: 115 kD
Application
Fig2: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Anei-ATX/Autotaxin Polyclonal Antibody, Unconjugated(ER1908-14) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
