Application
Fig1: Sample: FHC (human)Cell Lysate at 40 ug Primary: Anti- ENPP7 (ER1908-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 48 kDApplication
Fig2: Sample: HT29 (human)Cell Lysate at 40 ug Primary: Anti- ENPP7 (ER1908-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Product Name | ENPP7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALK SMase Alk-SMase Alkaline sphingomyelin phosphodiesterase E-NPP 7 Ectonucleotide pyrophosphatase/phosphodiesterase 7 Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 ENPP7 ENPP7_HUMAN Intestinal alkaline sphingomyelinase NPP-7. |
| Product description | Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human ENPP7 351-458/458 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ALK SMase Alk-SMase Alkaline sphingomyelin phosphodiesterase E-NPP 7 Ectonucleotide pyrophosphatase/phosphodiesterase 7 Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 ENPP7 ENPP7_HUMAN Intestinal alkaline sphingomyelinase NPP-7. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Note=Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. |
| SwissProt ID | Q6UWV6 |
|---|
Application
Fig1: Sample: FHC (human)Cell Lysate at 40 ug Primary: Anti- ENPP7 (ER1908-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 48 kD
Application
Fig2: Sample: HT29 (human)Cell Lysate at 40 ug Primary: Anti- ENPP7 (ER1908-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Positive Control | Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
