Application
Fig1: Protein: brain(mouse) lysate at 40ug; Primary: rabbit Anti-ENT2 (ER1908-16) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 50 kD Observed band size: 55 kDApplication
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti- ENT2 (ER1908-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Product Name | ENT2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DER12 Equilibrative NBMPR-insensitive nucleoside transporter Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter Equilibrative nucleoside transporter 2 HNP36 Hydrophobic nucleolar protein, 36 kDa SLC29A2 solute carrier family 29 (nucleoside transporters), member 29 Solute carrier family 29 member 2 S29A2_HUMAN. |
| Product description | ENT2 mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. It is less sensitive than ENT1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine. |
| Immunogen | KLH conjugated synthetic peptide derived from human ENT2 221-320/456 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DER12
Equilibrative NBMPR-insensitive nucleoside transporter
Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter
Equilibrative nucleoside transporter 2
HNP36
Hydrophobic nucleolar protein 36 kDa SLC29A2 solute carrier family 29 (nucleoside transporters) member 29 Solute carrier family 29 member 2 S29A2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Basolateral cell membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein. Note=Localized at the basolateral cell membrane in polarized MDCK cells. |
| SwissProt ID | Q14542 |
|---|
Application
Fig1: Protein: brain(mouse) lysate at 40ug; Primary: rabbit Anti-ENT2 (ER1908-16) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 50 kD Observed band size: 55 kD
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti- ENT2 (ER1908-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Positive Control | Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
