Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- Ep300 (ER1908-18) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 264 kD Observed band size: 264 kD| Product Name | Ep300 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREBBP/EP300 inhibitory protein 1 Cyclic AMP responsive enhancer binding protein E1A associated protein p300 E1A binding protein p300 E1A-associated protein p300 EC 2.3.1.48 EP300 EP300: E1A binding protein p300 EP300_HUMAN istone acetyltransferase p300 300 HAT RB and P300 binding protein EID 1 Retinoblastoma protein associated protein RSTS2. |
| Product description | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. |
| Immunogen | KLH conjugated synthetic peptide derived from human KAT3B/p300 1451-1600/2414 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CREBBP/EP300 inhibitory protein 1
Cyclic AMP responsive enhancer binding protein
E1A associated protein p300 E1A binding protein p300 E1A-associated protein p300 EC 2.3.1.48 EP300 EP300: E1A binding protein p300 EP300_HUMAN istone acetyltransferase p300 300 HAT RB and P300 binding protein EID 1 Retinoblastoma protein associated protein RSTS2. |
|---|---|
| Molecular Weight(MW) | 264 KDa |
| Cellular Localization | Cytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Co-localizes with ROCK2 in the nucleus. |
| SwissProt ID | Q09472 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- Ep300 (ER1908-18) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 264 kD Observed band size: 264 kD| Positive Control | Hela(Human) Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
