Application
Fig1: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-EP4 (ER1908-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kDApplication
Fig2: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-EP4 antibody (ER1908-19),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.Application
Fig3: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-EP4 (ER1908-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Product Name | EP4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prostaglandin E Receptor EP4 EP 4 EP4 EP-4 EP4R MGC126583 PE2R4_HUMAN PGE receptor EP4 subtype PGE2 receptor EP4 subtype Prostaglandin E receptor 4 Prostaglandin E receptor 4 subtype EP4 Prostaglandin E2 receptor Prostaglandin E2 receptor EP4 subtype Prostanoid EP4 receptor PTGER 2 PTGER 4 PTGER2 PTGER4. |
| Product description | Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. |
| Immunogen | KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4 151-250/488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanQueen Bee |
| Concentration | 1mg/ml |
| Alternative Names | Prostaglandin E Receptor EP4 EP 4 EP4 EP-4 EP4R MGC126583 PE2R4_HUMAN PGE receptor EP4 subtype PGE2 receptor EP4 subtype Prostaglandin E receptor 4 Prostaglandin E receptor 4 subtype EP4 Prostaglandin E2 receptor Prostaglandin E2 receptor EP4 subtype Prostanoid EP4 receptor PTGER 2 PTGER 4 PTGER2 PTGER4. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P35408 |
|---|
Application
Fig1: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-EP4 (ER1908-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD
Application
Fig2: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-EP4 antibody (ER1908-19),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Application
Fig3: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-EP4 (ER1908-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Positive Control | High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
