Application
Fig1: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EPHA10 Polyclonal Antibody, Unconjugated(ER1908-21) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- EPHA10 (ER1908-21) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107 kD| Product Name | EPHA10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPH receptor A10 EPHA 10 Epha10 EphA10s protein EPHAA_HUMAN Ephrin type A receptor 10 Ephrin type-A receptor 10. |
| Product description | Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5. |
| Immunogen | KLH conjugated synthetic peptide derived from human EPHA10 151-250/1008 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | EPH receptor A10 EPHA 10 Epha10 EphA10s protein EPHAA_HUMAN Ephrin type A receptor 10 Ephrin type-A receptor 10. |
|---|---|
| Molecular Weight(MW) | 107 KDa |
| Cellular Localization | Isoform 1, 3: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 2: Secreted (Probable). |
| SwissProt ID | Q5JZY3 |
|---|
Application
Fig1: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EPHA10 Polyclonal Antibody, Unconjugated(ER1908-21) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- EPHA10 (ER1908-21) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107 kD| Positive Control | Mainly expressed in testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
