Application
Fig1: Sample: Human colon carcinoma lysates Lysate at 30 ug Intestine (Mouse) Lysate at 30 ug Primary: Anti- EphA2/Eph receptor A2 (ER1908-22) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution Predicted band size : 105kD Observed band size : 105kD, 52/55kDApplication
Fig2: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-EphA2 (ER1908-22) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kD Observed band size: 115 kD| Product Name | EphA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARCC2 Eck Eph receptor A2 antibody EphA2 Ephrin receptor Ephrin receptor EphA2 Ephrin type A receptor 2 Epithelial cell kinase Epithelial cell receptor protein tyrosine kinase antibody Myk 2 Myk2 Sek 2 Sek2 Tyrosine protein kinase receptor ECK Tyrosine-protein kinase receptor MPK-5 Tyrosine-protein kinase receptor SEK-2 EPHA2_HUMAN. |
| Product description | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human EphA2 901-976/976 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowMonkey |
| Concentration | 1mg/ml |
| Alternative Names | ARCC2 Eck Eph receptor A2 antibody EphA2 Ephrin receptor Ephrin receptor EphA2 Ephrin type A receptor 2 Epithelial cell kinase Epithelial cell receptor protein tyrosine kinase antibody Myk 2 Myk2 Sek 2 Sek2 Tyrosine protein kinase receptor ECK Tyrosine-protein kinase receptor MPK-5 Tyrosine-protein kinase receptor SEK-2 EPHA2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 105 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Note=Present at regions of cell-cell contacts but also at the leading edge of migrating cells. |
| SwissProt ID | P29317 |
|---|
Application
Fig1: Sample: Human colon carcinoma lysates Lysate at 30 ug Intestine (Mouse) Lysate at 30 ug Primary: Anti- EphA2/Eph receptor A2 (ER1908-22) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution Predicted band size : 105kD Observed band size : 105kD, 52/55kD
Application
Fig2: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-EphA2 (ER1908-22) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kD Observed band size: 115 kD| Positive Control | Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
