Application
oxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EphA3) Polyclonal Antibody, Unconjugated (ER1908-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: H9C2(Rat) Cell Lysate at 30 ug Primary: Anti-EphA3 (ER1908-23) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 106 kD Observed band size: 106 kD| Product Name | EphA3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EK4 End3 Eph receptor A3 EPH-like kinase 4 EPH-like tyrosine kinase 1 EphA3 Ephrin receptor EphA3 Ephrin type-A receptor 3 ETK 1 ETK ETK1 HEK 4 HEK Hek4 Human embryo kinase Mek4 MGC109882 Receptor tyrosine kinase HEK Tyro 4 Tyro4 Tyrosine protein kinase receptor ETK 1 Tyrosine-protein kinase TYRO4 AW492086 Cek4 EC 2.7.10.1 EPHA3_HUMAN. |
| Product description | Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function. |
| Immunogen | KLH conjugated synthetic peptide derived from human EphA3/Eph receptor A3 401-500/983 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | EK4 End3 Eph receptor A3 EPH-like kinase 4 EPH-like tyrosine kinase 1 EphA3 Ephrin receptor EphA3 Ephrin type-A receptor 3 ETK 1 ETK ETK1 HEK 4 HEK Hek4 Human embryo kinase Mek4 MGC109882 Receptor tyrosine kinase HEK Tyro 4 Tyro4 Tyrosine protein kinase receptor ETK 1 Tyrosine-protein kinase TYRO4 AW492086 Cek4 EC 2.7.10.1 EPHA3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 106 KDa |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | P29320 |
|---|
Application
oxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EphA3) Polyclonal Antibody, Unconjugated (ER1908-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: H9C2(Rat) Cell Lysate at 30 ug Primary: Anti-EphA3 (ER1908-23) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 106 kD Observed band size: 106 kD| Positive Control | Widely expressed. Highest level in placenta. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
