Application
Fig1: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-EphA7 (ER1908-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/107 kD Observed band size: 52 kDApplication
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-EphA7 (ER1908-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/107 kD Observed band size: 52 kDApplication
Fig3: Protein: rat brain lysates, 30ug; Primary: Anti-EphA7 (ER1908-24) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 107kD Observed band size : 45kD| Product Name | EphA7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cek 11 Developmental kinase 1 EBK EHK-3 EHK3 EK11 Embryonic brain kinase Eph homology kinase 3 Eph receptor A7 EPH-like kinase 11 EphA7 EPHA7_HUMAN Ephrin receptor Eph A7 Ephrin type A receptor 7 Ephrin type-A receptor 7 hEK11 MDK 1 Receptor protein tyrosine kinase HEK 11 Tyrosine protein kinase receptor EHK 3. |
| Product description | ’Receptor tyrosine kinase which binds promiscuouslyGPI-anchored ephrin-A family ligands residing on adjacent cells,leading to contact-dependent bidirectional signaling intoneighboring cells. The signaling pathway downstream of the receptoris referred to as forward signaling while the signaling pathwaydownstream of the ephrin ligand is referred to as reversesignaling. Among GPI-anchored ephrin-A ligands, EFNA5 is acognate/functional ligand for EPHA7 and their interaction regulatesbrain development modulating cell-cell adhesion and repulsion. Hasa repellent activity on axons and is for instance involved in theguidance of corticothalamic axons and in the proper topographicmapping of retinal axons to the colliculus. May also regulate braindevelopment through a caspase(CASP3)-dependent proapoptoticactivity. Forward signaling may result in activation of componentsof the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 ANDMAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4which lacks the kinase domain may regulate isoform 1 adhesiveproperties. |
| Immunogen | KLH conjugated synthetic peptide derived from human EphA7 181-280/998 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Cek 11 Developmental kinase 1 EBK EHK-3 EHK3 EK11 Embryonic brain kinase Eph homology kinase 3 Eph receptor A7 EPH-like kinase 11 EphA7 EPHA7_HUMAN Ephrin receptor Eph A7 Ephrin type A receptor 7 Ephrin type-A receptor 7 hEK11 MDK 1 Receptor protein tyrosine kinase HEK 11 Tyrosine protein kinase receptor EHK 3. |
|---|---|
| Molecular Weight(MW) | 50/107 KDa |
| Cellular Localization | Cell membrane (Probable); Single-pass type I membrane protein (Probable). |
| SwissProt ID | Q15375 |
|---|
Application
Fig1: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-EphA7 (ER1908-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/107 kD Observed band size: 52 kD
Application
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-EphA7 (ER1908-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/107 kD Observed band size: 52 kD
Application
Fig3: Protein: rat brain lysates, 30ug; Primary: Anti-EphA7 (ER1908-24) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 107kD Observed band size : 45kD| Positive Control | Widely expressed in embryo. In adult, expression restricted to hippocampus, testis and spleen. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
