Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-EphB2 R (ER1908-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kDApplication
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated(ER1908-25) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated(ER1908-25) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | EphB2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Eph receptor B2 Developmentally regulated EPH related tyrosine kinase DRT ELK related protein tyrosine kinase EPH tyrosine kinase 3 EphB2 Ephrin type B receptor 2 EPHT 3 ERK ETECK Nuk Prkm 5 Receptor protein tyrosine kinase HEK 5, Sek 3 Tyro 5 Tyrosine protein kinase receptor CEK 5 Tyrosine protein kinase receptor EPH 3 Tyrosine protein kinase receptor QEK 5 EPHB2_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human EphB2 R 101-200/1055 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDog |
| Concentration | 1mg/ml |
| Alternative Names | Eph receptor B2
Developmentally regulated EPH related tyrosine kinase
DRT
ELK related protein tyrosine kinase
EPH tyrosine kinase 3
EphB2
Ephrin type B receptor 2
EPHT 3
ERK
ETECK
Nuk
Prkm 5
Receptor protein tyrosine kinase HEK 5 Sek 3 Tyro 5 Tyrosine protein kinase receptor CEK 5 Tyrosine protein kinase receptor EPH 3 Tyrosine protein kinase receptor QEK 5 EPHB2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 114 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite. |
| SwissProt ID | P29323 |
|---|
Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-EphB2 R (ER1908-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD
Application
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated(ER1908-25) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated(ER1908-25) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
