Application
Fig1: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- EphB6 (ER1908-26) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kDApplication
Fig2: Sample: U-2OS(Human) Cell Lysate at 30 ug Primary: Anti- EphB6 (ER1908-26) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD| Product Name | EphB6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPH receptor B6 EphB6 EPHB6_HUMAN Ephrin type B receptor 6 precursor Ephrin type-B receptor 6 HEP MGC129910 MGC129911 Tyrosine protein kinase defective receptor EPH 6 Tyrosine-protein kinase-defective receptor EPH-6. |
| Product description | Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. |
| Immunogen | KLH conjugated synthetic peptide derived from human EphB6 551-650/1021 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorse |
| Concentration | 1mg/ml |
| Alternative Names | EPH receptor B6 EphB6 EPHB6_HUMAN Ephrin type B receptor 6 precursor Ephrin type-B receptor 6 HEP MGC129910 MGC129911 Tyrosine protein kinase defective receptor EPH 6 Tyrosine-protein kinase-defective receptor EPH-6. |
|---|---|
| Molecular Weight(MW) | 111 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Isoform 3: Secreted. |
| SwissProt ID | O15197 |
|---|
Application
Fig1: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- EphB6 (ER1908-26) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD
Application
Fig2: Sample: U-2OS(Human) Cell Lysate at 30 ug Primary: Anti- EphB6 (ER1908-26) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD| Positive Control | Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
