Application
Fig1: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-Ephrin A5 (ER1908-27) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 23 kDApplication
Fig2: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Ephrin A5 (ER1908-27) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 23 kD| Product Name | Ephrin A5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPLG7 AL 1 EFNA5 EFL5 EPH related receptor tyrosine kinase ligand 7 LERK7 RAGS AF1 AL-1 Efna5 EFNA5_HUMAN EPH-related receptor tyrosine kinase ligand 7 Ephrin A5 Ephrin-A5 EPLG7 GLC1M LERK-7. |
| Product description | Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. |
| Immunogen | KLH conjugated synthetic peptide derived from human Ephrin A5 121-228/228 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | EPLG7 AL 1 EFNA5 EFL5 EPH related receptor tyrosine kinase ligand 7 LERK7 RAGS AF1 AL-1 Efna5 EFNA5_HUMAN EPH-related receptor tyrosine kinase ligand 7 Ephrin A5 Ephrin-A5 EPLG7 GLC1M LERK-7. |
|---|---|
| Molecular Weight(MW) | 24 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Membrane, caveola; Lipid-anchor, GPI-anchor. |
| SwissProt ID | P52803 |
|---|
Application
Fig1: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-Ephrin A5 (ER1908-27) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 23 kD
Application
Fig2: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Ephrin A5 (ER1908-27) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 23 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
