Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ermin) Polyclonal Antibody, Unconjugated (ER1908-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-Ermin (ER1908-31) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD| Product Name | Ermin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERMN ERM like protein Ermin ERMIN_HUMAN ermin, ERM-like protein JN Juxtanodin KIAA1189. |
| Product description | Plays a role in cytoskeletal rearrangements during the late wrapping and/or compaction phases of myelinogenesis as well as in maintenance and stability of myelin sheath in the adult. May play an important role in late-stage oligodendroglia maturation, myelin/Ranvier node formation during CNS development, and in the maintenance and plasticity of related structures in the mature CNS (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human Ermin 201-284/284 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | ERMN
ERM like protein
Ermin
ERMIN_HUMAN
ermin ERM-like protein JN Juxtanodin KIAA1189. |
|---|---|
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Cytoplasm; cytoskeleton |
| SwissProt ID | Q8TAM6 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ermin) Polyclonal Antibody, Unconjugated (ER1908-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-Ermin (ER1908-31) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD| Positive Control | Highly expressed in adult and fetal brain. Expressed at intermediate levels in the lung and liver. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
