Application
Fig1: Sample: SW480(Human) Cell Lysate at 40 ug Primary: Anti-ERO1L (ER1908-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Product Name | ERO1L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endoplasmic oxidoreductin 1 like protein Endoplasmic oxidoreductin-1-like protein ERO1 alpha ERO1 L ERO1 Lalpha ERO1 like protein alpha ERO1-L ERO1-L-alpha ERO1-like (S. cerevisiae) ERO1-like protein alpha ERO1A ERO1A_HUMAN ERO1L Oxidoreductin 1 Lalpha Oxidoreductin-1-L-alpha. |
| Product description | Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. |
| Immunogen | KLH conjugated synthetic peptide derived from human ERO1L 321-420/468 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Endoplasmic oxidoreductin 1 like protein Endoplasmic oxidoreductin-1-like protein ERO1 alpha ERO1 L ERO1 Lalpha ERO1 like protein alpha ERO1-L ERO1-L-alpha ERO1-like (S. cerevisiae) ERO1-like protein alpha ERO1A ERO1A_HUMAN ERO1L Oxidoreductin 1 Lalpha Oxidoreductin-1-L-alpha. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum. |
| SwissProt ID | Q96HE7 |
|---|
Application
Fig1: Sample: SW480(Human) Cell Lysate at 40 ug Primary: Anti-ERO1L (ER1908-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Positive Control | Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
